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strain |
supposed genotype |
ref allele |
mutation present? |
source |
parent strain? |
transcript on |
# exons |
mutation info |
sequencing dates |
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| TJ1109 |
age-1 (hx546) II |
none |
yes |
from Deqing (JC 9-21-5) |
TJ1052 x3 WB |
minus strand |
9 |
missense 806 pro to ser, CCA to TCA, bp 2416, exon 5 |
2-7-8-R-yes, 2-7-8-L-yes |
| TJ1101 |
clk-1 (e2519) III |
none |
no |
from Deqing (JC 9-26-5) |
CB4876 x20 WB |
plus strand |
5 |
missense 148 glutamic to lys, GAG to AAG, bp 442, exon 4 |
1-25-8-L-no, 1-29-8-R-no, 2-7-8-L-no |
| TJ1102 |
spe-10 (hc104) V |
none |
yes |
from Deqing (EG 4-25-4) |
BA744 no outcross |
plus strand |
4 |
nonsense 115 arg to opal stop, CGA to TGA, bp 343, exon 2 |
1-25-8-L-yes, 1-29-8-R-yes |
| TJ1103 |
daf-12 (m25) X |
m20 |
yes |
from Deqing (EG 4-30-4) |
DR25 no outcross |
plus strand |
17 |
missense 563 met to isoleu, ATG to ATA, bp 1689, exon 14 |
1-25-8-L-yes, 1-29-8-R-yes |
| TJ1104 |
daf-2 (e1370) III |
e1370 |
no |
from Deqing (JC 10-3-5) |
CB1370 no outcross |
minus strand |
17 |
missense 1462 pro to ser, CCA to TCA, bp 4384, exon 14 |
1-25-8-L-no, 1-29-8-R-no, 2-14-8-L-no, 2-18-8-R-no |
| TJ1105 |
daf-2 (e1370) III daf-12 (m25) X |
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daf-2 no, daf-12 yes |
from Deqing (EG 6-22-4) |
DR1337 not in WB |
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1-30-8-daf2R-no, 1-30-8-daf2L-no, 1-30-8-daf12R-yes, 1-30-8-daf12L-yes, 2-14-8-daf2L-no, 2-18-8-daf2R-no |
| TJ1111 |
age-1 (hx546) II spe-10 (hc104) V |
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age-1 yes, spe-10 yes |
from Deqing (JC 6-21-5) |
TJ909 not in WB |
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1-30-8-speL-yes, 1-30-8-speR-yes, 2-7-8-ageR-yes, 2-7-8-ageL-yes |
| CB4876a |
clk-1 (e2519) III |
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yes |
Forma II:1:15 (PT 10-18-91) |
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2-7-8-L-yes, 2-7-8-R-? |
| CB1370 |
daf-2 (e1370) III |
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no |
Forma I:5:20 (MK 5-16-92) |
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2-14-8-L-no, 2-18-8-R-no |
| DR1337 |
daf-2 (e1370) III daf-12 (m25) X |
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daf-2 yes, daf-12 yes |
Forma I:19:30 (EG 12-29-3) |
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2-14-8-daf2L-yes, 2-18-8-daf2R-yes, 2-18-8-daf2L-yes, 3-5-8-daf12L-yes, 3-5-8-daf12R-yes |
| TJ1100 |
clk-1 (qm30) III |
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qm30 del present |
Forma I:19:42 (by?) |
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590 bp del at AA 152, covers entire last exon, starts 12 bases 3' of e2519 |
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| TJ1101 |
clk-1 (e2519) III |
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qm30 del not present |
from Deqing (JC 9-26-5) |
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| TJ1109 |
age-1 (hx546) II |
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qm30 del not present |
from Deqing (JC 9-21-5) |
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| CB1370 |
daf-2 (e1370) III |
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yes |
from the CGC 2-25-8 |
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3-5-8-L-yes, 3-5-8-R-yes |
| CB4876 |
clk-1 (e2519) III |
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yes |
from the CGC 2-25-8 |
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3-5-8-L-yes, 3-5-8-R-yes |
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| Notes: |
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For the backcrossed strains which were not as expected, I repeated the sequencing starting from the worms given to me by Deqing. |
All sequences were done with both left and right primers except as noted. |
All sequencing was done starting with 10 or more worms and a "ten worm lysis prep". |
Primers were designed to produce a PCR amplicon of about 300bp spanning the allele of interest. |
Sequencing was performed on the amplicon using the same primers. |
The clk-1 (qm30) deletion was detected using PCR only and only done once. |
The parent strains were Shane's best guess. |