Jeremy Owens
Institute for Behavioral Genetics
Campus Box 447
University of Colorado at Boulder
Boulder, CO 80309-0447
Phone 303-492-2929
Fax 303-492-8063
email: JOWENS@COLORADO.EDU
Research Interests:
I am a first
year graduate student. My current research interests are
focused on the study
of ethanol sensitivity through the use of mouse
models. More specifically, I
am studying candidate genes for ethanol
sensitive behaviors by studying
knockout, transgenic, and congenic strains
of mice.
The congenic
mice were produced by crossing inbred long sleep mice
with inbred short sleep
mice. Mice that were LS for a particular region on
a chromosome were then
backcrossed for 10 generations to ISS mice, and
mice that were SS for a
particular region on a chromosome were then
backcrossed to ILS mice for 10
generations (Bennett & Johnson, 1998). I am
only currently interested in
studying the congenics that are LS for a
region on a SS chromosome.
The chromosomal regions referred to above have been named Lore1,
Lore2,
Lore4, and Lore5 (Lore stands for Loss Of Righting Reflex to
ethanol) which
are quantitative trait loci (QTLs) contributing to the
differences in loss of
righting reflex (LORR) between LS and SS mice. The
LORE QTLs were identified
using microsatellite markers on LSxSS F2 mice
(Markel et al., 1997).
I am using a variety of measures to examine differential ethanol
sensitivity
in the congenic strains of mice. These measures can be broken
down into four
different groups of tests.
1) Tolerance: These tests include both acute
and long-term tolerance.
Thelong-term tolerance is assessed by differential
LORR between two
hypnotic injections of ethanol given one week apart. Acute
tolerance is
assessed after exposure to low doses of ethanol. It is defined
as an
increase in performance on some task while blood ethanol
concentrations
are still increasing. The performance is typically measured
by amount of
time spent on a rotor rod.
2) Locomotor activation: This
is measured as the amount of activity
in an open field. Mice are given low
activating doses of ethanol before
they are placed in the open field
apparatus.
3) Ataxia: This is measured as the amount of time a mouse
can spend
on a rotor rod after being given a low dose of ethanol.
4)
Hypothermia: The temperature of the mice is taken prior to and
several times
after being given a hypnotic dose of ethanol.
In addition to testing
the congenics on these measures I am also
testing a variety of neurological
knockout mice for differential
sensitivity to ethanol. These neurological
knockouts are being tested for
tolerance, the activating effects of ethanol,
ataxia, hypothermia, and
LORR.