Mamm Genome 1994 Apr;5(4):199-202
Department of Psychology, CB 447 University of Colorado, Boulder 80309-0447.
We are mapping quantitative trait loci (QTLs) that influence ethanol-induced anesthesia (sleep time) in the Long-sleep (LS) and Short-sleep (SS) selected lines of mice. Fifty microsatellite-STS markers were initially screened for simple-sequence length polymorphisms between the LS and SS lines. Nineteen markers were polymorphic. Eleven markers unequivocally differentiated the LS and SS lines and were used to establish strain distribution patterns for the LSXSS series of recombinant inbred strains. Five markers each accounted for at least 5% of sleep-time genetic variance among the RI strains. Linkage of provisional QTLs detected among RIs will be confirmed or disproved in a large F2 population. This ongoing QTL-mapping project eventually will result in a strain distribution pattern for the LSXSS RI series with an average marker spacing of 5 centimorgans.